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DiAGSure® Hepatitis D Viral Load Kit
Features
Sample Type: Human Plasma, Serum samples
Fast: The extraction process completes in 1 hr 10 mins
Compatibility: The DiAGSure® Hepatitis D Viral Load Kit is compatible with a broad range of real-time PCR platforms
Specificity: The target sequences detected in this kit specifically targets the Hepatitis D Structural antigen (HDAg) gene of HDV
Sensitivity: The analytical sensitivity of the assay was obtained using a dilution series of quantified in-vitro transcribed HDAg RNA spiked in negative plasma sample as a template for detection
Precision: Precision was calculated based on intra-assay and inter-assay variability of the lowest copy number kit quantitative standard (HDV Standard 4) for HDV detection (FAM channel).
Applications:
- Screening and Diagnosis
- Research and Epidemiology
- Public Health Programs
- Point-of-Care Testing
Categories:
- A Hepatitis Detection Kit is categorized as an in vitro diagnostic (IVD) medical device used for detecting markers of hepatitis viruses in patient samples, aiding in disease diagnosis and monitoring.
Product :
DiAGSure® Hepatitis D Viral Load Kit
Catalog No :
GDQ2354- 25R; GDQ2354- 50R; GDQ2354-100R
#Description
Hepatitis D is an inflammation of the liver caused by the hepatitis D virus (HDV), which requires HBV for its replication. Hepatitis D infection cannot occur in the absence of hepatitis B virus. HDV-HBV co-infection is considered the most severe form of chronic viral hepatitis due to more rapid progression towards hepatocellular carcinoma and liver-related death. The routes of HDV transmission, like HBV, occur through broken skin (via injection, tattooing etc.) or through contact with infected blood or blood products. Transmission from mother to child is possible but rare. Vaccination against HBV prevents HDV coinfection and hence expansion of childhood HBV immunization programs has resulted in a decline in hepatitis D incidence worldwide. Chronic HBV carriers are at risk of infection with HDV. People who are not immune to HBV (either by natural disease or immunization with the hepatitis B vaccine) are at risk of infection with HBV, which puts them at risk of HDV infection.
#Specification
The HDV PCR assay can detect Hepatitis D virus and is a much more sensitive and fast detection method over conventional antibody-based and histopathological methods for the detection of viral infection or for describing the natural history of viral infection. The DiAGSure® Hepatitis D Viral Load Kit is an in vitro diagnostic (IVD) real-time polymerase chain reaction (qPCR) test involving TaqMan chemistry intended for the detection of Hepatitis D viral RNA in human clinical and viral culture samples. The kit can detect HDV genotypes 1-8. This two-plex PCR detects the Hepatitis D Structural antigen (HDAg) gene target of the viral genome and includes an endogenous beta-actin internal control (IC) reference gene in a single-tube reaction.
#Kit Content
| Cap | Contents | Description | GDQ2354-25R | GDQ2354-50R | GDQ2354-100R |
| Red | UNG+ OS Master Mix | Amplification Mix | 100 µL | 240 µL | 500 µL |
| Amber | Primer-Probe Mix | Amplification Reagent | 60 µL | 120 µL | 250 µL |
| Yellow | HDV Standard 1 | Quantification Standard | 50 µL | 100 µL | 200 µL |
| Yellow | HDV Standard 2 | Quantification Standard | 50 µL | 100 µL | 200 µL |
| Yellow | HDV Standard 3 | Quantification Standard | 50 µL | 100 µL | 200 µL |
| Yellow | HDV Standard 4 | Quantification Standard | 50 µL | 100 µL | 200 µL |
| Blue | Negative Control (NC) | Negative Control | 250 µL | 500 µL | 1 mL |
| White | RNA Dilution Buffer | Tris-based buffer | 250 µL | 500 µL | 1 mL |
#Viral Load Estimation
There are four quantification standards provided with the kit with viral RNA copies as follows:
| Standard | Copies/μL |
| HDV Standard 1 | 104 |
| HDV Standard 2 | 103 |
| HDV Standard 3 | 102 |
| HDV Standard 4 | 101 |
A plot of Ct value vs RNA copies of the standards will fetch an equation wherefrom the viral copy number of an unknown sample can be deduced from its Ct value. The quantification standards exhibit linearity when Ct values are plotted against log (copies/ μL) with R2 value>0.99. The efficiency of amplification should be above 80% with the slope of the standard curve in the range -3.1 to -3.8.
The quantitation standards are defined as RNA copies/μL. The following equation has to be applied to convert the values determined using the standard curve into copies/ml of sample material:
Frequently Asked Questions
Our Price
25 Reaction
₹ POR
50 Reaction
₹ POR
100 Reaction
₹ POR
Product Brochure
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Product Data Sheet
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Contact Us
- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
