GLyse® RIPA (Radio-Immune Precipitation Assay) buffer is one of the most reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. GLyse® RIPA Buffer does not contain any kind of inhibitors. If desired, add protease inhibitors, phosphatase inhibitors, kinase inhibitors immediately before use.