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GSure® Soil DNA Isolation Kit
High-efficiency extraction of microbial & environmental DNA from complex soil matrices
The GSure® Soil DNA Isolation Kit is a robust, inhibitor-removal–optimized system designed to extract high-quality DNA from all types of soil, sludge, sediment, compost, and environmental particulate samples. Soil is among the most challenging sample types due to humic substances, polyphenols, heavy metals, and organic inhibitors. The GSure® kit combines mechanical disruption, chemical lysis, and multi-step inhibitor elimination to deliver PCR-ready, inhibitor-free DNA ideal for molecular biology and metagenomic applications.
This kit is suitable for research laboratories, agricultural biotechnology, environmental monitoring, microbial ecology, biodiversity studies, and industrial soil analysis workflows.
Key Features & Benefits (USP)
● Superior Inhibitor Removal System: Multi-stage extraction with GSD4 and GSD5 buffers efficiently eliminates humic acids, polyphenols, and other PCR inhibitors—common problems in soil DNA isolation.
● High Yield & High Purity DNA: Optimized lysis with GSD1–GSD3 buffers ensures strong recovery of bacterial, fungal, viral, and microbial community DNA from even the most challenging soil types.
● Silica Column–Based Purification: GMini Spin Columns allow fast, consistent, and high-quality DNA purification.
● Suitable for All Downstream Applications: Purified DNA performs reliably in PCR, qPCR, metagenomics, NGS, DGGE, cloning, sequencing, and community profiling.
● Fast, Streamlined Workflow: Complete DNA isolation in ~40 minutes using standard centrifuge equipment.
● Industry-Grade Performance at an Economical Cost: Comparable output to leading global kits (e.g., Qiagen, Macherey-Nagel) at significantly lower cost.
● Stable Storage Conditions: Buffers GSD3 and GSD4 stored at 4°C; all other components stored at room temperature for easy handling.
Applications
━ Soil microbial ecology
━ Environmental DNA (eDNA) analysis
━ Agriculture, plant-microbe research
━ Soil biodiversity studies
━ Bioremediation monitoring
━ Industrial soil quality testing
━ Detection of soil-borne pathogens
Product:
GSure® Water DNA Isolation Kit
Catalog No :
G4444, G4444A, G4444B
Description
The GSure® Soil DNA Isolation Kit provides a complete, optimized workflow for extracting DNA from:
━ Agricultural soil
━ Clay, sandy soil, loamy soil
━ Compost, peat & humus-rich samples
━ Sewage sludge, sediments, and industrial waste soil
━ Forest soil with high organic content
The workflow
━ Sample lysis (GSD1–GSD3)
━ Two-step inhibitor removal (GSD4 & GSD5)
━ DNA binding using GSD6
━ Column purification
━ Elution in nuclease-free water
The datasheet (page 1) presents the complete protocol including incubation, vortexing, centrifugation speeds, and volume handling.
The resulting DNA is clean, concentrated, and free of humic inhibitors, making it directly suitable for all molecular applications.
Workflow Parameters
| Parameter | Specification |
| Sample Type | Soil, sludge, sediment, compost, particulate matter |
| Sample Quantity | 250 mg per extraction |
| Lysis Method | Chemical + mechanical disruption (vortexing with GSD1 vial) |
| Inhibitor Removal | Dual-stage using GSD4 & GSD5 |
| Purification Method | Silica spin column |
| Max Centrifuge Speed | 12,000 × g |
| Elution Volume | 50–100 μL (100 μL recommended) |
| Storage | GSD3 & GSD4 at 4°C; others at RT |
Why Choose GSure® Soil DNA Isolation Kit?
Soil is one of the most complex biological matrices for DNA extraction. The GSure® system solves these challenges with:
● Strong lysis chemistry
● Rigorous inhibitor removal
● High-yield recovery
● Dependable reproducibility
This makes GSure® the preferred choice for microbial ecology, environmental genomics, agricultural R&D, and industrial microbiology.
#Our Key Advantage
Unique Advantages Compared to Competing Soil DNA Kits
● Designed for humic-rich Indian soil conditions, where global kits often underperform
● Highly effective inhibitor removal, enabling direct PCR without additional cleanup
● Greater DNA recovery from tough samples (clay, contaminated soil, peat)
● Optimized for environmental microbiome workflows
● Economical without compromising on quality
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Contact Us
- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
