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GSure® Water DNA Isolation Kit
Rapid, high-yield purification of microbial and environmental DNA from water samples.
The GSure® Water DNA Isolation Kit is an optimized silica-spin–column based extraction system designed for efficient recovery of high-quality DNA from environmental water samples. Whether the goal is microbial surveillance, environmental monitoring, sewage/wastewater testing, or pathogen detection, this kit ensures robust lysis, efficient inhibitor removal, and high-purity DNA, suitable for downstream applications including PCR, qPCR, metagenomics, and next-generation sequencing (NGS).
The protocol is designed to process filtered water samples using mechanical lysis with glass beads, followed by chemical extraction and silica-column purification. The kit performs reliably across a wide range of water sources—groundwater, surface water, industrial effluent, treated water, and environmental monitoring samples.
Key Features & Benefits (USP)
● High DNA Yield from Low Biomass Samples: Efficient lysis using glass bead–based disruption + high-stringency extraction buffers, ensuring maximum recovery even from low microbial load water samples.
● Removes PCR Inhibitors Effectively: Proprietary buffers (GDW1, GDW2 & GDW3) remove humic acids, salts, detergents, and environmental contaminants, enabling inhibition-free PCR/qPCR.
● Silica Column–Based Purification: GMini Spin Columns ensure clean, intact DNA ideal for molecular detection workflows.
● Compatible With All Downstream Applications: Purified DNA performs robustly in PCR, qPCR, digital PCR, metagenomics, sequencing, cloning, RFLP, etc.
● User-Friendly, Fast Protocol: Complete extraction within 35–40 minutes, using standard microcentrifuge—no toxic chemicals, no phenol/chloroform.
● Room-Temperature Storage: All reagents are stable at room temperature, enabling convenient laboratory use.
● Flexible Elution: Adjustable elution volume (50–200 µL) for concentration needs.
Applications
━ Environmental surveillance
━ Water quality testing
━ Wastewater-based epidemiology
━ Detection of bacteria, viruses & microbial communities
━ Industrial/municipal water monitoring
━ Research in microbiology & metagenomics
Product:
GSure® Water DNA Isolation Kit
Catalog No :
G4612, G4613, G46131, G4615
Description
The GSure® Water DNA Isolation Kit provides a complete workflow for extracting environmental DNA from up to 100 mL filtered water samples. The protocol incorporates:
━ Mechanical disruption of trapped microbes using glass beads
━ High-efficiency lysis using GDW1 buffer
━ Contaminant removal using GDW2 and GDW3 buffers
━ DNA capture and purification on GMini silica columns
━ High-quality elution using nuclease-free water
The method enables reproducible isolation of genomic DNA from bacteria, fungi, viral particles retained on membrane filters, and mixed microbial populations. Each reagent has been validated for environmental DNA applications.
Detailed step-by-step extraction workflow, buffer behavior, wash conditions, and elution guidelines are provided in the kit’s official datasheet.
Workflow Parameters
| Parameter | Specification |
| Sample Type | Filtered water sample (0.2 µm membrane) |
| Lysis Method | Glass bead–based mechanical disruption + chemical lysis |
| Purification Method | Silica spin column |
| Maximum Spin Speed | 10,000 × g |
| Elution Volume | 50–200 µL (recommended 100 µL) |
| DNA Quality | PCR-ready, inhibitor-free |
| Typical Time Required | 35–40 minutes |
| Storage Condition | Room Temperature |
Why Choose GSure® Water DNA Isolation Kit?
If your application demands speed, purity, and reproducibility, GSure® technology ensures dependable extraction across a wide variety of environmental matrices. The kit is widely used in:
● Environmental microbiology laboratories
● Public health surveillance networks
● Municipal sewage monitoring systems
● Research institutes and biotech R&D labs
● QC/QA laboratories for water treatment facilities
A robust workflow, optimized chemistry, and consistent performance make GSure® an ideal choice for water DNA isolation.
#OVERVIEW
Unique Advantages Over Competing Kits (Qiagen-Style Comparison)
● More economical while delivering comparable yield and purity
● Room-temperature stable—no cold-chain dependency
● Fast, simplified workflow without hazardous chemicals
● Highly efficient bead-beating step pre-integrated (others often require external homogenizers or kits)
● Designed for real-world Indian environmental conditions (turbidity, mixed microbial load)
● Reliable results even from extremely low-biomass water samples
Product Data Sheet
Access detailed specifications, features, and technical information in PDF format.
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