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GSure® Plant DNA Isolation Kit (with WLN Buffer)
High-efficiency extraction of genomic DNA from complex plant matrices
The GSure® Plant DNA Isolation Kit with WLN Buffer is a robust, inhibitor-removal–optimized system designed to extract high-quality genomic DNA from diverse plant tissues, including fresh leaves, roots, seeds, flowers, and lyophilized samples. Plant tissues contain polysaccharides, polyphenols, pigments, and secondary metabolites, which significantly inhibit PCR and reduce DNA yield. The GSure® kit combines optimized chemical lysis, dual-buffer softening, Proteinase K digestion, and silica column purification to deliver PCR-ready, inhibitor-free DNA ideal for plant molecular biology, genetic analysis, and biotechnology workflows.
This kit is suitable for agricultural R&D, plant breeding programs, biotechnology laboratories, phylogenetic studies, environmental research, and genome analysis workflows.
Key Features & Benefits (USP)
● Superior Inhibitor Removal System: The combined action of WLN Buffer, PB Buffer, RW1 Buffer, and AMD Buffer efficiently eliminates polysaccharides, polyphenols, pigments, tannins, and other PCR inhibitors typically abundant in plant samples.
● High Yield & High Purity DNA: Optimized lysis with pre-warmed WLN+PB+RW1 mix and Proteinase K ensures strong recovery of plant genomic DNA from even tough tissues.
● Silica Column–Based Purification: GMini Spin Columns provide fast, consistent, and high-quality DNA purification.
● Suitable for All Downstream Applications:
Purified DNA performs reliably in:
PCR, qPCR, sequencing, cloning, genotyping, DNA barcoding, restriction digestion, and NGS workflows.
● Fast, Streamlined Workflow: Complete DNA isolation in ~30 minutes using standard centrifuge equipment.
● Industry-Grade Performance at an Economical Cost: Comparable output to leading global kits (Qiagen DNeasy Plant Mini, Thermo GeneJET Plant DNA Kit) at significantly lower cost.
● Stable Storage Conditions: All components can be stored at room temperature except Proteinase K (4°C).
Applications:
- Plant molecular genetics
- PCR/qPCR amplification from plant genomic DNA
- DNA barcoding & phylogenetic analysis
- Agriculture, plant-microbe & transgenic research
- Biodiversity studies & ecological surveys
- Detection of plant pathogens
- NGS library preparation from plant DNA
Product :
DNA Gel Loading Dye
Catalog No :
G4620S, G4620M, G4620L
#Descriptions
The GSure® Plant DNA Isolation Kit provides a complete, optimized workflow for extracting genomic DNA from:
Fresh leaf tissue (80–100 mg) Lyophilized plant tissue (up to 20 mg) Roots, seeds, stems, flowers, and metabolite-rich samples Plant species containing high levels of inhibitors
The workflow incorporates:
● Sample Lysis (Pre-warmed WLN + PB + RW1 mix, Proteinase K)
Tissue is crushed (with or without liquid nitrogen), treated with the preheated buffer mixture, and incubated at 65°C for enhanced breakdown of plant cell walls.
● Inhibitor Removal (AMD Buffer)
AMD Buffer precipitates pigments, polysaccharides, and polyphenols, ensuring maximal inhibitor removal and clear lysate formation.
● DNA Binding Using RP1 Buffer
The clarified lysate is mixed with RP1 Binding Buffer and loaded onto the GMini DNA Binding Column.
● Column Purification
Washing with Membrane Wash Buffer removes residual contaminants.
● Elution in Nuclease-Free Water
The resulting DNA is clean, concentrated, and inhibitor-free, making it directly suitable for all molecular applications.
The datasheet provides complete stepwise handling, incubation times, and centrifugation conditions.
#Technical Specifications
Workflow Parameters
| Parameter | Specification |
| Sample Type | Leaf, seed, root, flower, lyophilized plant tissue |
| Sample Quantity | 80–100 mg fresh tissue; ≤20 mg dried tissue |
| Lysis Method | WLN + PB + RW1 (preheated) + Proteinase K |
| Inhibitor Removal | AMD Buffer |
| Purification Method | Silica spin column |
| Max Centrifuge Speed | 12,000 × g |
| Elution Volume | 50–100 µL |
| Storage | Proteinase K at 4°C; all other buffers at RT |
Unique Advantages Compared to Competing Plant DNA Kits
● Specifically designed for polysaccharide- and polyphenol-rich Indian plant samples
● Highly effective inhibitor removal enabling direct PCR without cleanup
● Greater DNA recovery from tough or metabolite-rich tissues
● Optimized for plant genetics & molecular biotechnology workflows
● Economical without compromising on DNA purity or performance
Why Choose GSure® Plant DNA Isolation Kit?
Plant tissues are among the most complex biological matrices for DNA extraction. The GSure® system overcomes these challenges with:
● Strong multi-buffer lysis chemistry
● Rigorous inhibitor removal
● High-yield recovery
● Robust reproducibility
This makes GSure® the preferred choice for plant biotechnology, agricultural genomics, species authentication, and advanced R&D applications.
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