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GSure® Sputum DNA Isolation Kit
Reliable, fast, and high-yield genomic DNA extraction from sputum samples
The GSure® Sputum DNA Isolation Kit is designed to efficiently extract high-quality genomic DNA from freshly collected sputum samples, including thick, mucous-rich, and clinically processed specimens. Sputum contains viscous mucopolysaccharides, cell debris, protein aggregates, and PCR inhibitors that make extraction challenging. The GSure® system overcomes these obstacles using a three-buffer lysis cascade (GDSP1 → GDSP2 → GDSP3) optimized for viscosity reduction, mucolytic action, and effective DNA release.
Purified DNA is suitable for PCR, qPCR, sequencing, genotyping, and molecular diagnostic assay development—especially in TB, respiratory pathogen, and clinical microbiology workflows.
Key Features & Benefits
● Optimized for Difficult Sputum Samples: The sequential action of GDSP1, GDSP2, and GDSP3 at 70°C ensures effective mucolysis, cell disruption, and protein denaturation. This is critical for thick, mucoid sputum samples.
● High-Purity PCR-Ready DNA: Column-based purification using GMini Spin Columns removes salts, proteins, mucopolysaccharides, and lysis residues—yielding DNA suitable for sensitive downstream assays.
● Flexible Sample Collection Options
Handles:
200–300 µL freshly collected sputum, or Swab-collected specimens directly processed using GDSP1 buffer
● Fast Workflow (~20–25 minutes after lysis): Simple lysis → bind → wash → elute procedure enables efficient processing even in high-throughput diagnostic environments.
● No Organic Extraction Required: Completely avoids phenol/chloroform, ensuring user safety and cleaner DNA.
● Room-Temperature Stable Kit Components: All buffers and columns are stable at ambient conditions—ideal for Indian logistics and field laboratories.
● Economical Alternative to Premium Global Kits: Delivers comparable performance to leading sputum DNA kits at significantly lower operational cost.
Applications:
- DNA extraction for respiratory pathogen testing
- Mycobacterial DNA isolation workflows
- PCR / qPCR assay development
- Sequencing & genotyping
- Clinical microbiology R&D
- Public health and infectious disease laboratories
- Molecular epidemiology studies
Product :
DNA Gel Loading Dye
Catalog No :
G45273A, G45273, G45273B
#Descriptions
The GSure® Sputum DNA Isolation Kit uses a multi-stage lysis system tailored for viscous sputum, followed by silica-column purification.
● Sample Lysis Add 250 µL GDSP1 to 200–300 µL sputum → incubate at 70°C for 15 minutes, vortex every 2 minutes. For swab samples: immerse swab in 300 µL GDSP1, roll and press against tube wall. Add 250 µL GDSP2, invert gently and incubate again at 70°C for 15 minutes. Add 350 µL GDSP3, mix by inversion (no vortexing). Centrifuge 10 minutes @ 13,000 rpm to pellet debris.
● Binding to Column Transfer supernatant carefully to GMini Spin Column (avoid pellet). Spin at 13,000 rpm for 30–60 seconds and discard flow-through.
● Washing Add 600 µL Membrane Wash Buffer and spin. Repeat wash. Perform an additional 2-minute dry spin to remove ethanol—critical for enzymatic reactions.
● Elution Add 50 µL Nuclease-Free Water to center of the membrane. Stand for 1 minute. Spin 1 minute @ 13,000 rpm to elute purified DNA
Technical Specifications
| Parameter | Specification |
| Sample Type | Fresh sputum or swab-collected sputum |
| Input Volume | 200–300 µL |
| Lysis Buffers | GDSP1, GDSP2, GDSP3 |
| Incubation Temp | 70°C (multiple steps) |
| Binding Method | Silica spin column |
| Wash Steps | 2 washes + dry spin |
| Elution Volume | 50 µL |
| Storage | Room temperature |
| Total Prep Time | ~20–25 minutes (post lysis) |
Why Choose GSure® Sputum DNA Isolation Kit?
● Specifically optimized for thick, mucous-rich sputum
● High-quality DNA ideal for TB, respiratory pathogen, and clinical PCR workflows
● Fast, reliable, easy to scale in diagnostic or research setups
● Far more economical than international sputum DNA kits
● Fully room-temperature stable—ideal for Indian conditions
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- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
