DNA Biology
- Home
- GSure® Gel Extraction Kit
GSure® Gel Extraction Kit
Fast, high-recovery DNA purification from agarose gels with built-in pH indicator
The GSure® Gel Extraction Kit enables rapid purification of DNA fragments from agarose gels using an optimized GGMB gel-melting buffer supported by a pH-sensitive colour indicator system. This indicator ensures the correct pH for optimal DNA binding, improving recovery efficiency and preventing sample loss.
Designed with silica spin-column technology, the kit produces DNA suitable for all downstream applications, including cloning, sequencing, PCR, qPCR, ligation, and restriction digestion. The streamlined melt → bind → wash → elute workflow makes this kit ideal for routine molecular cloning and research laboratories.
Gel slices dissolve efficiently at 60°C, and complete colour-guided pH adjustment ensures consistent results across all gel conditions. The GSure® Gel Extraction Kit provides a reliable, fast, and simple solution for laboratory DNA purification needs.
Key Features & Benefits
● High DNA Recovery
Efficient extraction of DNA fragments from agarose gel slices up to 300 mg, suitable for fragments between 70 bp – 10 kb.
● Integrated pH Indicator (Color-Coded Buffer System)
GGMB buffer visually indicates optimal conditions for DNA binding:
● Straw yellow → Ideal pH for silica binding
● Pink → High pH; adjust with sodium acetate (pH 5.2)
This prevents poor recovery due to incorrect pH.
● Fast Gel Dissolution
Complete melting of gel slices within 10–15 minutes at 60°C, ensuring rapid sample processing.
● Silica Column–Based Purification
GMini spin columns selectively bind DNA while removing salts, polysaccharides, agarose, and dye contaminants.
● High-Quality DNA for Downstream Applications
Purified DNA is compatible with:
PCR, qPCR, sequencing, ligation, restriction digestion, cloning, and other enzymatic workflows.
● Simple, User-Friendly Protocol
Minimal steps, no organic solvents, and only a basic microcentrifuge required.
● Room-Temperature Storage
All buffers and columns can be stored at ambient conditions, offering convenience in Indian laboratory environments.
Applications:
- Recovery of DNA fragments from agarose gels
- Purification of DNA for cloning & subcloning workflows
- Ligation and restriction digestion reactions
- PCR and qPCR template preparation
- Sequencing and mutagenesis workflows
- Removing impurities after enzymatic reactions
Product :
DNA Gel Loading Dye
Catalog No :
G4629A, G4629B, G4629C
#Descriptions
GSure® Gel Extraction Kit
The GSure® Gel Extraction Kit provides a reliable and efficient method for extracting high-purity DNA from agarose gels using color-guided pH adjustment and silica-column purification.
Gel Dissolution
Gel slices (≤300 mg) are excised and incubated with 3× volume GGMB buffer at 60°C until completely dissolved. GGMB contains a pH indicator that signals optimal binding conditions:
● Pink → high pH; adjust with 3M sodium acetate (pH 5.2)
● Straw yellow → correct pH for binding
Add 1 gel volume isopropanol, mix, and briefly centrifuge. DNA Binding
The melted mixture is loaded onto the GMini silica column and centrifuged. DNA binds to the membrane while contaminants flow through. Washing
Columns are washed twice using ethanol-supplemented Membrane Wash Buffer, followed by a dry spin to remove residual ethanol—critical for downstream enzymatic reactions. Elution
Purified DNA is eluted with 50 µL nuclease-free water, generating DNA ready for cloning and other applications.
Detailed handling and centrifugation conditions are included in the official datasheet.
Technical Specifications
| Parameter | Specification |
| Sample Type | Agarose gel slice (≤300 mg) |
| Fragment Size Range | ~70 bp – 10 kb |
| Gel Dissolution Temperature | 60°C (10–15 minutes) |
| pH Indicator | Present in GGMB buffer (pink/yellow) |
| Binding Method | Silica spin-column |
| Wash Steps | 2 washes (ethanol added) |
| Elution Volume | 50 µL nuclease-free water |
| Total Time Required | 15–20 minutes |
| Storage Condition | All components at room temperature |
Why Choose GSure® Gel Extraction Kit?
The GSure® Gel Extraction Kit is the ideal choice for laboratories requiring fast, reliable, and high-yield DNA purification directly from agarose gels.
It is widely used in:
● Molecular cloning and genetic engineering
● DNA fragment purification for sequencing
● PCR product cleanup
● Enzymatic reaction workflows
● Academic, research, biotech & diagnostic laboratories
The color-guided pH system, efficient gel melting chemistry, and dependable silica-column performance make GSure® a trusted solution for gel-based DNA recovery needs.
Related Products
Send Your Query to Us
Contact Us
- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
