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G-Bond Ni-NTA Agarose Bead
High-capacity, low-leakage Ni-NTA resin for purification of His-tagged recombinant proteins
G-Bond Ni-NTA Agarose Resin is a high-performance, crosslinked agarose matrix charged with nitrilotriacetic acid (NTA)–chelated nickel ions (Ni²⁺) for highly selective purification of 6×His-tagged recombinant proteins under both native and denaturing conditions.
Engineered to match or outperform leading MNC products (Qiagen, Thermo Fisher, Sigma, Cytiva), G-Bond Ni-NTA provides superior binding capacity, exceptional mechanical stability, minimal nickel leakage, and excellent reusability, making it ideal for research labs, production facilities, and bioprocess development workflows.
Key Features & Benefits
● Industry-leading binding capacity (40–60 mg His-tag protein/mL resin)
Significantly higher than many standard Ni-NTA resins (Qiagen: 20–30 mg/mL; Thermo HisPur: 30 mg/mL). Ideal for high-yield purification and scale-up.
● Very low nickel (Ni²⁺) leakage
Highly stable NTA–Ni chelation ensures minimal Ni²⁺ bleed-through, improves purity, and protects downstream enzymatic reactions.
● Highly crosslinked Superflow-grade agarose beads
Provides excellent flow uniformity, mechanical strength, and compatibility with gravity columns, batch purification, and low-pressure FPLC.
● Works under both native and denaturing conditions Compatible with:
8 M Urea 6 M Guanidine-HCl Reducing agents Detergents High-salt buffers
● Reusable up to 20–25 cycles without loss of performance
Regeneration and recharging protocols allow long-term economic use.
● Superior clearance of host-cell proteins & contaminants
Highly selective Ni²⁺–His binding reduces nonspecific protein contamination, delivering highly pure elution fractions.
● Economical & high-performance alternative to MNC resins
Delivers global-standard purification at significantly reduced cost.
Applications:
- E. coli
- Yeast
- Insect cells
- Mammalian cells
Product :
G-Bond Ni-NTA Agarose Bead
Catalog No :
GPC-01A, GPC-01B, GPC-01C, GPC-01D
#Buffer Recommendations
Binding Buffer
50–150 mM Sodium phosphate (pH 7.5–8.0)
300 mM NaCl
10–20 mM imidazole
Wash Buffer
20–40 mM imidazole
Optional: 0.1–0.5% nonionic detergents, 10% glycerol
Elution Buffer
250–300 mM imidazole
Alternative elution: pH gradient (pH 5.0–4.0)
Compatibility With Common Additives
| Additive | Compatibility |
| Urea (up to 8 M) | ✔ Compatible |
| Guanidine-HCl (up to 6 M) | ✔ Compatible |
| β-mercaptoethanol | ✔ Compatible |
| DTT | ✔ Compatible up to 8 mM |
| EDTA, EGTA | ✘ Not recommended (chelates Ni²⁺) |
| Detergents (Triton X-100, Tween 20, NP-40) | ✔ Compatible |
| High-salt buffers | ✔ Fully compatible |
#Regeneration & Recharging Protocol
Regeneration
- Wash with 10 column volumes of water
- Wash with 50 mM EDTA (to remove Ni²⁺)
- Wash thoroughly with water
- Recharge
Recharging
- Incubate resin with 100 mM NiSO₄
- Wash with water
- Store in 20% ethanol
Resin retains 95–100% binding capacity after 20 regeneration cycles.
Example SDS-PAGE (add more pics)
Clear single-step purification
High purity in elution fractions
Minimal host-protein background
Ordering Information
| Product | Description | Pack Size |
| G-Bond Ni-NTA Agarose Resin | High-capacity Ni-NTA agarose for His-tag purification | 10 mL, 25 mL, 100 mL, Bulk with catalogue number |
Technical Specifications
| Parameter | G-Bond Ni-NTA Resin |
| Matrix | Highly crosslinked agarose (Superflow equivalent) |
| Ligand | Nitrilotriacetic acid (NTA) |
| Metal ion | Ni²⁺ |
| Bead size | 25–165 µm |
| Binding capacity | 15–25 mg His-tag protein/mL resin |
| Max. linear flow rate | ≥ 300 cm/h (gravity/FPLC) |
| pH stability | pH 3–12 |
| Storage buffer | 20% ethanol |
| Reusability | 20–25 cycles |
| Recommended formats | Batch, gravity-flow, FPLC (low pressure) |
Why Choose G-Bond Ni-NTA?
● Higher yield per mL compared to global brands
● Superflow-grade bead strength for reproducibility
● Cost-effective without compromising quality
● Ideal for Indian research, biotech & manufacturing environments
● Backed by GCC Biotech service & supply assurance
G-Bond Ni-NTA vs Leading MNC Ni-NTA Resins
| Feature | G-Bond Ni-NTA | Qiagen Ni-NTA | Thermo HisPur | Sigma His•Bind |
| Binding Capacity | ⭐ 15–25 mg/mL | 20–30 mg/mL | 30 mg/mL | 20–40 mg/mL |
| Ni²⁺ Leakage | ⭐ Ultra-low | Low | Medium | Medium |
| Reusability | ⭐ 20–25 cycles | 5–10 cycles | 3–10 cycles | 5–8 cycles |
| Bead Strength | High | High | Medium | Medium |
| Denaturing Compatibility | Full (6 M GuHCl / 8 M Urea) | Full | Full | Full |
| Flow Properties | Excellent | Excellent | Good | Good |
| Cost Efficiency | ⭐ Outstanding | Expensive | High | High |
Conclusion:
G-Bond Ni-NTA matches or outperforms Qiagen, Thermo, and Sigma in binding capacity, nickel stability, reusability, and cost effectiveness.
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Contact Us
- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
