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G-Quant BCA Protein Assay Kit
Sensitive, detergent-compatible colorimetric protein quantification (BCA method)
The G-Quant BCA Protein Assay Kit is a ready-to-use, bicinchoninic acid–based colorimetric assay for rapid and reliable determination of protein concentration in a wide range of sample types. The assay is compatible with most detergent-containing lysis buffers, offers a broad linear range from 0.1–2.5 mg/mL, and can be completed in less than 20 minutes using either microplate or cuvette formats.
Designed as a cost-effective alternative to leading BCA kits from global brands, G-Quant maintains comparable sensitivity and robustness while providing superior convenience and room-temperature reagent stability.
How the Assay Works
The BCA assay is based on the reduction of Cu²⁺ to Cu⁺ by peptide bonds and specific amino acid residues (cysteine, cystine, tryptophan, tyrosine) under alkaline conditions. Bicinchoninic acid (BCA), a water-soluble chromogenic reagent, chelates Cu⁺ to form a purple complex with strong absorbance at 562 nm. The intensity of this color is directly proportional to the protein concentration in the sample.
G-Quant BCA is optimized to produce a stable, high-contrast signal with minimal interference from commonly used buffer components.
Key Features & Benefits
● Wide working range: 0.1–2.5 mg/mL
Quantifies low to high protein concentrations from a single standard curve using only 10 µL sample per well.
● Detergent-compatible
Validated for use with commonly used lysis buffers containing detergents, where Lowry/Bradford assays often fail.
● Fast protocol – < 20 minutes total time
Incubation of only 10 minutes at 37°C with gentle shaking; ideal for high-throughput workflows.
● Room-temperature stable reagents
Both Reagent A (500 mL) and Reagent B (10 mL) are stable at RT, simplifying storage & shipping and giving G-Quant an edge over kits that require cold-chain logistics.
● Flexible formats: plate or cuvette
The same chemistry can be used in 96-well plates (10 µL sample + 200 µL reagent) or cuvettes (100 µL sample + 2 mL reagent), allowing seamless scale-up from screening to detailed analysis.
● Ready-to-use working solution
Simple 50:1 mixing of Reagent A:B generates the working solution; any transient precipitate dissolves on mild shaking — no extra buffers or additives needed.
● Comprehensive troubleshooting guidance
The kit documentation provides clear solutions for low signal, high background, and interfering substances, making it friendly even for new users.
Applications:
- Quantification of total protein in:
● Cell lysates
● Tissue extracts
● Purified protein samples
● Column fractions (Ni-NTA, ion-exchange, etc.)
● Membrane and detergent-solubilized proteins - Standardization of sample loading for SDS-PAGE, Western blot, ELISA
- Enzyme activity normalization
Product :
G-Quant BCA Protein Assay Kit
Catalog No :
GPQ10, GPQ11
#Descriptions
Competitive Positioning vs. MNC BCA Kits
Compared with typical BCA kits from large multinationals (Thermo/Pierce, Sigma, etc.), G-Quant BCA offers:
| Attribute | G-Quant BCA | Typical MNC BCA Kits* |
| Working range | 0.1–2.5 mg/mL | Similar |
| Sample volume (plate) | 10 µL | 5–25 µL |
| Incubation | 10 min at 37°C | 20–30 min at 37–60°C |
| Detergent compatibility | Optimized for common lysis buffers | Similar |
| Reagent stability | Room temperature | Often 2–8°C recommended |
| Format flexibility | Plate & cuvette protocols provided | Plate/cuvette, often separate SKUs |
| Cost per assay | Lower (Make-in-India) | Higher (imported) |
*Typical specifications based on publicly available BCA kit descriptions; exact values vary by brand and catalog.
Bottom line: G-Quant matches the sensitivity and robustness of global BCA assays while providing shorter incubation time, RT-stable reagents, and significantly better cost efficiency—ideal for routine use and high-throughput screening in Indian labs.
Interference & Troubleshooting
The kit documentation includes detailed troubleshooting notes, for example:
Low protein signal → Increase sample volume, extend incubation (up to 60 min) and/or temperature (up to 60°C). Interfering substances → Prepare standards in the same buffer, or clean samples using GCC Protein Clean-up Kit (Cat. No. GPQ 20S). High blank / purple color without protein → Indicates presence of reducing agents or thiols; clean up or modify the sample. No color development → Buffers may contain strong Cu-chelators; dilute/desalt or use clean-up kit.
This guidance helps users obtain consistent results even with challenging sample matrices.
Why Choose G-Quant BCA?
● Performance comparable to leading BCA kits from Thermo/Pierce, Sigma, etc.
● Reagent stability at room temperature, ideal for Indian lab conditions.
● Shorter incubation time with strong, stable color development.
● High tolerance to detergents commonly used in lysis buffers.
● Excellent value for money, enabling routine and large-scale use without compromising data quality.
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