DiAGSure® Sickle Cell Anemia Detection Kit

Intent to use:
The DiAGSure® Sickle Cell Anemia Detection Kit is a real-time PCR assay developed for the accurate detection of the single nucleotide polymorphism (SNP) in the β-globin gene (HBB) that results in sickle cell anemia. This specific A→T transversion at codon 6 leads to the substitution of valine for glutamic acid, producing abnormal hemoglobin S (HbS). The structural change in red blood cells causes them to assume a rigid, sickle shape—leading to vaso-occlusion, chronic anemia, pain crises, and organ damage. The kit enables precise genotyping of this SNP, distinguishing between homozygous disease (HbSS), heterozygous carriers (HbAS), and normal individuals. Early detection is essential for newborn screening, prenatal diagnostics, and population-based carrier programs. With high sensitivity and specificity, the DiAGSure® kit supports timely clinical decisions and genetic counseling in regions where hemoglobinopathies are prevalent.

Key Specifications:
 Targets the A→T SNP in the HBB gene, responsible for sickle cell anemia.
 Accurately differentiates between HbSS, HbAS, and normal genotypes using real-time PCR.
 Supports early diagnosis and carrier detection, crucial for preventive healthcare planning.
 High analytical sensitivity and specificity ensure dependable genotyping from genomic DNA.
 Seamless compatibility with major qPCR platforms, facilitating integration into clinical workflows

Sample types:
Whole Blood, EDTA Plasma.

Product information: