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DiAGSure® Hepatitis A Virus TaqMan PCR kit
Features
Sample Type: Human Plasma, Serum samples
Fast: The extraction process completes in 1 hr 20 mins
Compatibility: The DiAGSure® Hepatitis A Viral Load Kit is compatible with a broad range of real-time PCR platforms
Specificity: The target sequences detected in this kit specifically targets the 5’-UTR regions of Hepatitis A Virus (HAV)
Detection Limit: The absolute sensitivities were obtained using a dilution series of quantified synthetic RNA (in-vitro transcribed) as a template for detection
Safety: The DiAGSure® Hepatitis A Virus TaqMan PCR kit is for laboratory use only
Applications:
- Screening and Diagnosis
- Research and Epidemiology
- Public Health Programs
- Point-of-Care Testing
Categories:
- A Hepatitis Detection Kit is categorized as an in vitro diagnostic (IVD) medical device used for detecting markers of hepatitis viruses in patient samples, aiding in disease diagnosis and monitoring.
Product:
DiAGSure® Hepatitis A Virus TaqMan PCR kit
Catalog No:
GDQ6054- 20R; GDQ6054- 50R; GDQ6054-100R
#Description
The DiAGSure® Hepatitis A Virus TaqMan PCR kit PCR assay can detect HAV and is a much more sensitive and fast detection method for the detection of viral infection. The DiAGSure® Hepatitis A Virus TaqMan PCR kit (Multiplex) is an in vitro diagnostic (IVD) Real-time Reverse Transcriptase-polymerase chain reaction (qRT-PCR) test involving TaqMan chemistry intended for the detection of viral RNA in plasma and serum samples. This two-plex PCR detects the 5’ Untranslated region (UTR) of the viral genome and includes an internal control (IC) reference gene in a single-tube reaction.
#Specification
Hepatitis A virus (HAV) is a highly contagious RNA virus belonging to the Picornaviridae family, known for causing acute liver inflammation and hepatitis. Discovered in 1973, HAV is primarily transmitted through the fecal-oral route, typically via ingestion of contaminated food or water or through close personal contact with an infected individual. The virus primarily targets hepatocytes in the liver, leading to symptoms such as jaundice, fatigue, abdominal pain, nausea, and fever. While most cases of HAV infection resolve spontaneously within a few weeks with supportive care, severe complications such as acute liver failure can occur, especially in older adults or individuals with underlying liver disease. Vaccination against HAV is highly effective in preventing infection and is recommended for individuals at increased risk of exposure, travellers to endemic areas, and certain high-risk groups.
#Ct Cut-Off
| Target | Cut-off Ct | Interpretation |
| Viral gene – 5’UTR (FAM) | Ct≤40 | Viral RNA positive |
| IC (Cy5) | Ct≤35 | Internal control positive |
#Kit Content
| Color Coding (Caps) | Reagents | Description | GDQ6054-20R (20 tests) | GDQ6054-50R (50 tests) | GDQ6054-100R (100 tests) |
| Red | 2X One-step Master Mix | Amplification Mix | 300 µL | 700 µL | 1.4 mL |
| Amber | Primer-Probe Mix | Amplification Reagent | 40 µL | 100 µL | 200 µL |
| Yellow | Positive Control (PC) | Positive Control | 20 µL | 50 µL | 100 µL |
| Blue | Negative Control (NC) | Negative Control | 200 µL | 500 µL | 1 mL |
| White | RNA Dilution Buffer | Tris-based buffer | 200 µL | 500 µL | 1 mL |
Frequently Asked Questions
Our Price
20 Isolation
₹ POR
50 Isolation
₹ POR
100 Isolation
₹ POR
Product Brochure
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Product Data Sheet
Access detailed specifications, features, and technical information in PDF format.
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Contact Us
- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
