Sample Type: Human Plasma, Serum samples
Fast: The extraction process completes in 1 hr 20 mins
Compatibility: The DiAGSure® Hepatitis E Viral Load Kit is compatible with a broad range of real-time PCR platforms
Specificity: The target sequences detected in this kit specifically targets a conserved region in the capsid gene of HEV.
Detection Limit: The absolute sensitivities were obtained using a dilution series of quantified synthetic RNA (in-vitro transcribed) as a template for detection
Safety: The DiAGSure® Hepatitis E Virus TaqMan PCR kit is for laboratory use only
Hepatitis E is an infectious disease of the liver caused by Hepatitis E Virus (HEV); it is a type of viral hepatitis. Symptoms of hepatitis E range from Fatigue, sudden nausea and vomiting, abdominal pain or discomfort diarrhea, dark-coloured urine and jaundice (a yellowing of the skin and whites of the eyes). Not everyone who is infected will have all of the symptoms.
The DiAGSure® Hepatitis E Virus TaqMan PCR kit PCR assay can detect HEV and is a much more sensitive and fast detection method for the detection of viral infection. The DiAGSure® Hepatitis E Virus TaqMan PCR kit (Multiplex) is an in vitro diagnostic (IVD) Real-time Reverse Transcriptase-polymerase chain reaction (qRT-PCR) test involving TaqMan chemistry intended for the detection of viral RNA in human plasma and serum samples. This two-plex PCR detects the HEV capsid gene and includes an endogenous RNase-P internal control (IC) in a single-tube reaction.
Target | Cut-off Ct | Interpretation |
Viral gene (HEX/VIC) | Ct≤40 | Viral RNA positive |
IC (Cy5) | Ct≤35 | Internal control positive |
Color Coding (Caps) | Reagents | Description | GDQ2100-20R (20 tests) | GDQ2100-50R (50 tests) | GDQ2100-100R (100 tests) |
Amber | 2X one-step Master Mix | Amplification Mix | 300 µL | 700 µL | 1.4 mL |
Amber | Primer-Probe Mix | Amplification Reagent | 40 µL | 100 µL | 200 µL |
Yellow | Positive Control (PC) | Positive Control | 20 µL | 50 µL | 100 µL |
White | Negative Control (NC) | Negative Control | 200 µL | 500 µL | 1 mL |
White | RNA Dilution Buffer | Tris-based buffer | 200 µL | 500 µL | 1 mL |
20 Reaction
₹ POR
50 Reaction
₹ POR
100 Reaction
₹ POR
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