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GSure® Plasmid DNA Isolation Kit
Rapid, high-purity plasmid DNA purification with visual lysis indication
The GSure® Plasmid Mini Kit enables fast and efficient purification of high-quality plasmid DNA from E. coli cultures using an alkaline lysis method supported by a unique Sheer PINK chromogenic indicator. This indicator allows users to visually track each lysis and neutralization phase, ensuring consistent sample processing and improved reproducibility.
Designed using silica spin-column technology, the kit produces plasmid DNA suitable for all molecular downstream applications, including restriction digestion, PCR, qPCR, cloning, sequencing, and in-vitro enzymatic reactions. The system is optimized to deliver high yield from standard culture volumes while maintaining purity levels comparable to premium global kits.
The workflow combines controlled alkaline lysis, precise neutralization, and efficient column-based purification, making it ideal for routine molecular biology as well as research-intensive laboratories.
Key Features & Benefits
High Yield Plasmid Recovery
Efficient extraction of plasmid DNA from 5–10 mL overnight E. coli cultures, suitable for high-, mid-, and low-copy plasmids.
Real-Time Visual Lysis Control (Sheer PINK Indicator)
A built-in chromogenic dye provides visual confirmation at each step:
● Pink suspension → lysis initiated
● Clear pink solution → complete lysis
● Colorless cloudy lysate → full neutralization
This prevents under- or over-lysis and improves reproducibility.
Silica Column–Based Purification
GMini spin columns bind plasmid DNA selectively while removing RNA, proteins, salts, and genomic DNA contaminants.
Downstream Compatibility
Purified plasmid DNA is suitable for:
PCR, qPCR, sequencing, restriction digestion, cloning, ligation, mutagenesis, and in vitro transcription.
Fast, User-Friendly Protocol
A complete miniprep can be finished within 20–25 minutes using only a standard microcentrifuge—no phenol, chloroform, or hazardous chemicals.
Convenient Storage
Only GSPB1 requires 4°C storage; all other reagents are stable at room temperature, making the kit ideal for Indian laboratory conditions.
Flexible Elution
Standard elution uses 50 µL nuclease-free water; low-volume or pre-warmed elution can increase concentration when required.
Applications:
Routine plasmid miniprep Cloning and subcloning workflows PCR & qPCR template preparation Restriction digestion and mapping Sanger sequencing Mutagenesis and plasmid engineering Research in molecular biology & synthetic biology
Product :
GSure® Plasmid DNA Isolation Kit
Catalog No :
G46121, G4613, G46131
#Descriptions
The GSure® Plasmid DNA Isolation Kit provides a robust alkaline lysis workflow followed by silica-column purification to recover highly pure plasmid DNA.
● Resuspension
Complete dispersal of the bacterial pellet using GSPB1 ensures uniform lysis. Avoid visible clumps to prevent genomic DNA contamination. (GSPB1 must be stored at 4°C.)
● Alkaline Lysis + Sheer PINK Indicator
Addition of GSPB2 initiates lysis, turning the solution pink. Gentle inversion (no vortexing) ensures controlled lysis. A clear pink solution indicates complete lysis.
● Neutralization
GSPB3 is added, turning the mixture colorless and cloudy, marking complete neutralization and precipitation of cell debris, genomic DNA, and proteins.
● Clarification
Centrifugation pellets debris; the cleared lysate is carefully loaded onto the GMini column.
● DNA Binding and Washing
The plasmid binds to the silica membrane; subsequent wash steps with ethanol-enriched Membrane Wash Buffer ensure removal of salts and contaminants.
● Elution
Plasmid DNA is eluted using 50 µL nuclease-free water, producing clean, ready-to-use DNA for molecular workflows.
Detailed step-by-step handling, buffer usage, and centrifugation conditions are provided in the kit’s official datasheet.
Technical Specifications
| Parameter | Specification |
| Sample Type | Overnight E. coli culture |
| Recommended Culture Volume | 5–10 mL |
| Lysis Method | Alkaline lysis with Sheer PINK visual indicator |
| Purification Method | Silica spin-column |
| Maximum Spin Speed | ~12,000 × g |
| Elution Volume | 50 µL (adjustable) |
| DNA Quality | Restriction digestion–ready, PCR-ready |
| Typical Time Required | 20–25 minutes |
| Storage Condition | GSPB1 at 4°C; others at RT |
Why Choose GSure® Plasmid DNA Isolation Kit?
If your workflow demands speed, reproducibility, and reliable plasmid yields, the GSure® Plasmid Mini Kit provides the ideal balance of performance and affordability.
It is widely used in:
● Academic and research laboratories
● Biotech R&D and molecular cloning labs
● Synthetic biology groups
● QC/QA units
● Teaching laboratories and training facilities
The optimized buffer chemistry, visual indicator system, and consistent performance make GSure® a dependable choice for everyday plasmid purification.
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