Reagent
Within 2 Working Day
Fast– Isolation completes within 30 min
Convenient- Wide range of starting culture volume (1ml-12ml)
High yield- Recovers 3μg plasmid DNA from 1ml culture
Reproducible- Equal amount of yield on every isolation
Easy to use- Unique chromogenic lysis indicator sheerPink
Quality of DNA- 260:280 absorbance ratio stays around 1.79-1.83. No contamination of protein and/or RNA and/or gDNA
Wide Range- Can isolate plasmid sized from 2.6kb-18kb
Downstream application- more than 75% isolated plasmid DNA comes in ccc form, best for downstream application
Cost effective– More preps for the money
G4615
25 Isolation
₹ POR
G4616
100 Isolation
₹ POR
G4617
10 Isolation
₹ POR
G4618
25 Isolation
₹ POR
G4619
100 Isolation
₹ POR
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Ut elit tellus, luctus nec ullamcorper mattis, Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
Ihgfyj dsfg: Ut elit tellus, luctus
Riout nbvf: Ut elit tellus, luctus
Fel hbjew: Ut elit tellus, luctus
Boki Cebcxzqwe: Ut elit tellus, luctus
Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
Inpoi: Ut elit tellus, luctus
Reqewr tuiop: Ut elit tellus, luctus
Fecxzdas lkjyu: Ut elit tellus, luctus
Brtycvb: Ut elit tellus, luctus
GSure Plasmid DNA Isolation Kit is an alkaline lysis based plasmid DNA isolation kit which employs silica membrane mini spin column for rapid and fast purification of the isolated DNA molecule. This kit can isolate covalently closed circular (ccc) plasmid DNA molecule from fresh overnight grown E.coli cells. Isolated plasmid is free from genomic DNA/Protein or RNA contamination. Isolation of plasmid DNA completes within 30 min. The kit can isolate at least 3μg plasmid DNA from 1ml of overnight grown culture of high copy number plasmid. For low copy number plasmid, yield generally varies from 500ng-1.5μg from 1ml culture. Yield could also be enhanced by chloramphenicol. GSure Plasmid DNA Isolation Kit can isolate plasmid DNA from 1ml to 12 ml of overnight grown cells. More than 75% of the isolated plasmid DNA is in ccc form which is the best suitable form of plasmid DNA molecule for any sort of downstream application. Most ccc form of plasmid DNA found upto 3ml of starting culture volume. More than 3ml culture does not inhibit plasmid DNA isolation, but the amount of ccc form of DNA decreases.
Gsure Plasmid DNA Isolation kit can isolate different sized plasmid DNA with equal efficiency. 2.6 kb plasmid to 18kb plasmid DNA molecule has been isolated by the kit efficiently. Isolated plasmid DNA is free of genomic DNA. A PCR reaction using 16S rDNA specific primer pair was set up on isolated plasmid DNA molecule, generated no amplification establishing that the plasmid DNA is free of trace amount of contaminating gDNA. Spectrophotometric absorbance data reveals that the isolated plasmid DNA is free of protein and RNA contamination. A260:A280 ratio varies between 1.79-1.83. Plasmid DNA isolated by GSure Plasmid DNA isolation kit is compatible for all sorts of downstream application like restriction digestion, PCR amplification, Sanger sequencing or in vitro transcription. GSure Plasmid DNA Isolation kit comes with a unique chromogenic indicator, sheerPink. SheerPink helps to ensure completion of lysis reaction properly. A complete lysis and proper neutralization assures ccc form of isolated plasmid and no genomic DNA contamination. GSure Plasmid DNA Isolation Kit contains 3 buffers which assure complete lysis of cells and digestion of contaminating proteins. Wash buffer supplied with the kit confirms complete removal of proteins and contaminants from the silica membrane. Optimized protocol guaranties extremely purified plasmid DNA isolation reproducibly. All the buffers should be stored at room temperature. Prescribed volume of absolute ethanol is to be added with the wash buffer before starting the work.
Quality Control System
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et
Lorem ipsum dolor
Lorem ipsum dolor sit amet, consetetur sadip scing elitr, sed diam nonumy
Fermentum purus
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy
Product Size | 500U & 2500U |
Shipping Condition: | Dry Ice |
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem
Lorem ipsum dolor sit amet, consectetur adipi
Lorem ipsum dolor sit amet, consectetur adipi
Lorem ipsum dolor sit amet, consectetur adipi
Lorem ipsum dolor sit amet, consectetur adipi
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt