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GSure® Urine DNA Isolation Kit
Sensitive, high-purity isolation of cell-free and exfoliated genomic DNA from urine samples
The GSure® Urine DNA Isolation Kit is a silica-spin–column–based nucleic acid purification system optimized for efficient extraction of cell-free DNA (cfDNA), exfoliated epithelial cell DNA, and microbial DNA from fresh or processed urine samples. Its unique chemistry ensures maximum recovery of low-abundance DNA species typically present in urine, while removing PCR inhibitors such as salts, urea, metabolic by-products, and urinary pigments.
This kit is designed for early-stage molecular diagnostics, cancer biomarker research, UTI and microbial DNA detection, transplant rejection monitoring, and various clinical research workflows that rely on high-sensitivity detection of urine-derived nucleic acids.
Key Features & Benefits
● High Recovery of Low-Abundance Urine DNA
Optimized binding chemistry ensures superior extraction of cell-free DNA and trace-level genomic DNA—ideal for mutation detection, infectious disease diagnostics, and biomarker analysis.
● Effective Removal of Urinary PCR Inhibitors: Unique buffer system efficiently removes contaminants such as salts, urea, proteins, and metabolites, providing PCR-ready DNA without additional cleanup.
● Silica Column–Based Purification: GMini columns offer high binding capacity with consistent DNA recovery across sample types, including dilute or low-volume urine samples.
● Fast, Easy, User-Friendly Protocol: Complete extraction in 35–40 minutes using a standard microcentrifuge. No phenol, chloroform, or hazardous reagents.
● Room-Temperature Reagent Storage: All kit components are stable at room temperature, simplifying transport, storage, and routine laboratory use.
● Flexible Input & Elution Volume: Works with 0.5–10 mL urine (pre-processed). Elution volume can be adjusted (50–200 µL) depending on DNA concentration needs.
Applications:
- Cell-free DNA analysis
- Cancer biomarker research (liquid biopsy)
- Urinary tract infection (UTI) microbiology
- Viral & microbial DNA detection
- Transplant rejection monitoring
- Genetic and epigenetic studies
- Research in molecular diagnostics
Product :
GSure® Urine DNA Isolation Kit
Catalog No :
G46372A, G46372B, G46372E
#Descriptions
The GSure® Urine DNA Isolation Kit provides a complete workflow for isolating high-quality DNA from urine—one of the lowest-biomass clinical samples used in molecular biology. The kit incorporates:
● Efficient lysis of exfoliated epithelial cells and microbial cells using optimized buffers
● Selective binding of cfDNA and genomic DNA to silica matrix
● Removal of PCR-inhibitory compounds commonly found in urine
● High-purity elution suitable for sensitive downstream applications
The workflow is designed to ensure reproducibility across samples with variable volume, pH, turbidity, and nucleic acid content. Each reagent is validated for clinical research and molecular diagnostic workflows that require high purity and sensitivity.
Technical Specifications
| Parameter | Specification |
| Sample Type | Fresh urine, stored urine, or centrifuged urine pellet |
| Sample Input Volume | 0.5–10 mL |
| Lysis Method | Chemical lysis with optimized GU buffers |
| Purification Method | Silica spin-column (GMini) |
| Maximum Spin Speed | 10,000 × g |
| Elution Volume | 50–200 µL |
| DNA Quality | PCR-ready, inhibitor-free DNA |
| Typical Time Required | 35–40 minutes |
| Storage Condition | Room Temperature |
Unique Advantages Over Competing Kits Higher cost-efficiency compared with
● Room-temperature reagent stability, eliminating the cold chain and logistical challenges often associated with imported kits.
● Simplified workflow requiring no magnetic racks, vacuum manifolds, or specialized instruments.
● Optimized for Indian sample conditions, including variable pH, low nucleic acid content, and high metabolic inhibitor load.
● Flexibility in handling different urine volumes, unlike several competitor kits that require fixed sample inputs or additional concentration steps.
Why Choose GSure® Urine DNA Isolation Kit?
The GSure® Urine DNA Isolation Kit is an ideal solution for laboratories working on:
● Molecular diagnostics
● Clinical research
● Biomarker and oncology programs
● Microbiology and infectious disease studies
● High-throughput screening projects
● Academic and biotechnology R&D
With its reliable chemistry, high sensitivity, and consistent performance, GSure® delivers dependable urine DNA extraction for even the most demanding applications.
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