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GSure® Plant RNA Isolation Kit
High-purity total RNA extraction from plant tissues rich in polysaccharides & secondary metabolites
The GSure® Plant RNA Isolation Kit is a uniquely engineered system for extracting high-quality, inhibitor-free total RNA from a broad variety of plant tissues—including samples rich in polysaccharides, polyphenols, secondary metabolites, and enzymatic contaminants that typically interfere with RNA purification.
Using an optimized buffer system (GRP1 + Part A/B + GRP2 + GRP3) and a dual-column strategy (DNA removal followed by RNA binding), the kit overcomes challenges commonly encountered with plant RNA extraction and consistently delivers RNA suitable for RT-PCR, qPCR, RNA-Seq, Northern blotting, and downstream gene-expression assays.
Key Features & Benefits
● Designed Specifically for Plant Complexity
The dual-chemistry formulation (Part A for general plants; Part B for metabolite-rich plants like tea, neem, bamboo, pine) ensures effective RNA isolation even when working with high-polyphenolic or mucilage-rich tissues.
● Complete Removal of DNA & Plant-Derived Contaminants
An initial pass through the GMini DNA Binding Column eliminates genomic DNA and high-mass debris, enabling clean RNA binding in the subsequent step.
● High-Purity RNA for Sensitive Downstream Applications
Two-step washing removes polysaccharides, phenolics, ethanol, and inhibitory pigments that commonly cause failure in qPCR and enzymatic reactions.
● Efficient at Small Sample Input
Works efficiently with 20–30 mg fresh leaf tissue—ideal for labs with limited sample availability.
● Temperature-Stable Reagents
All components are stable at room temperature, simplifying storage and transport.
● No Phenol-Chloroform Required
A safer, cleaner alternative to TRIzol-based methods while offering comparable RNA quality.
Applications:
- RT-PCR and RT-qPCR
- RNA-Seq and transcriptomics
- Gene expression profiling
- Plant stress physiology studies
- Viral and pathogen RNA detection in crops
- Agricultural biotechnology research
- Transgenic and genome-editing programs
Product :
DNA Gel Loading Dye
Catalog No :
GR1004A, GR1004, GR1004B
#Descriptions
The GSure® Sputum DNA Isolation Kit uses a multi-stage lysis system tailored for viscous sputum, followed by silica-column purification.
● Sample Lysis
Add 250 µL GDSP1 to 200–
The GSure® Plant RNA Isolation Kit uses a combination of mechanical disruption, heat-assisted lysis (70°C), selective precipitation, DNA removal, and silica-column binding to generate total RNA of exceptional quality.
The workflow includes:
● GRP1 + Part A/B for efficient lysis Part A: For most plant tissues Part B: For plants with high polyphenol/secondary metabolite content
GR1004_GSure® Plant RNA Isolati…
● GRP2 & GRP3 for selective solubilization and precipitation
This step eliminates pigments, polysaccharides, tannins, and inhibitors.
● GMini DNA Binding Column for DNA & debris removal
Flow-through is enriched with RNA.
● GMini RNA Binding Column for final purification
Ensures high-quality RNA recovery suitable even for ultra-sensitive downstream reactions.
Overall runtime: 35–45 minutes.
Technical Specifications
| Parameter | Specification |
| Sample Type | Fresh plant leaf tissue (20–30 mg) |
| Lysis Chemistry | GRP1, Part A/B, GRP2, GRP3 |
| Pre-Purification | GMini DNA Binding Column |
| Purification Method | GMini RNA Binding Column (silica-based) |
| Elution Volume | 50 µL nuclease-free water |
| Total Isolation Time | ~40 minutes |
| RNA Output | High-quality total RNA (A260/280: 1.8–2.0) |
| Storage | Room temperature for all reagents |
| Downstream Uses | RT-PCR, qPCR, sequencing, blotting, cloning |
The GSure® Plant RNA Isolation Kit offers unmatched performance for complex plant tissues, delivering:
● High yield
● Exceptional purity
● Reproducibility across species
● Ease of use
● Competitive pricing
● Compatibility with all expression and sequencing workflows
This makes it the preferred choice for agricultural biotechnology, academic laboratories, genomics research, and crop diagnostic programs.
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- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
