#Descriptions

Phenol-chloroform-isoamyl alcohol (IAA) reagents are a specialized mixture used in molecular biology laboratories for the efficient extraction and purification of nucleic acids, particularly DNA and RNA, from various biological samples. The reagent, a combination of phenol, chloroform, and isoamyl alcohol in specific ratios, creates a biphasic solution that effectively separates nucleic acids in the upper aqueous phase from proteins and cellular debris in the lower organic phase. This mixture is available in neutral pH for DNA extraction or acidic pH for optimal RNA preservation. The resulting high-quality nucleic acids are suitable for various downstream applications.

#Specification

Phenol-chloroform-isoamyl alcohol (IAA) reagents are specialized mixtures designed for the extraction and purification of nucleic acids, such as DNA and RNA, from biological samples. Typically consisting of a specific ratio of phenol, chloroform, and isoamyl alcohol, these reagents facilitate phase separation during nucleic acid extraction. The biphasic mixture formed enables efficient isolation of nucleic acids in the upper aqueous phase while proteins and cellular debris partition into the lower organic phase. These reagents offer a choice of neutral pH for DNA extraction or acidic pH (~4-5) for optimal RNA preservation. High-quality nucleic acids obtained using these reagents are suitable for various downstream applications

#OVERVIEW

Kit Content

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