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MOPS Buffer
Features
- • MOPS is a Good’s buffer with a pKa around 7.2 at 25°C, making it an ideal buffer for maintaining a stable, near-neutral pH suitable for RNA electrophoresis
- • The buffer helps optimize the separation of RNA molecules by providing an appropriate environment that supports the denaturation of RNA and prevents secondary structure formation during electrophoresis
- • The buffer is supplied as a 10X concentrated solution, allowing for easy dilution to a working concentration of 1X as needed, providing convenience and flexibility in preparation
- • The buffer is typically RNase-free, minimizing the risk of RNA degradation during electrophoresis and ensuring the integrity of RNA samples
- • The buffer provides consistent ionic strength and conductivity for efficient RNA migration and separation in gel electrophoresis
Categories:
- RNA Electrophoresis
- Facilitating RNA integrity
- Visualization of RNA
- Formaldehyde-Agarose Gels
Applications:
- 10X and 1X MOPS Buffer fall under the category of electrophoresis buffers for RNA analysis
Product :
MOPS Buffer
Catalog No :
GCR-C4, GCR-C4A, GCR-E3, GCR-E3A,
#Description
10X MOPS Buffer is a concentrated solution of 3-(N-morpholino)propanesulfonic acid (MOPS) designed for use in RNA electrophoresis, specifically for formaldehyde-agarose gels. This buffer provides a stable pH environment (around 7.2 at 25°C), optimizing the separation of RNA molecules based on size and maintaining their integrity during electrophoresis. The 10X concentration allows easy dilution to the working concentration of 1X, offering convenience and flexibility in preparation. MOPS Buffer is also RNase-free, ensuring the protection of RNA samples from degradation and preserving high-quality results. It delivers consistent ion conductivity for efficient RNA migration and compatibility with various electrophoresis systems and dyes for clear visualization. Proper storage and handling maintain the buffer’s stability and effectiveness, making it an essential tool for researchers in molecular biology and related fields conducting RNA analysis for research and diagnostic applications.
#Specification
10X MOPS Buffer is a concentrated solution of 3-(N-morpholino)propanesulfonic acid (MOPS) designed for use in RNA electrophoresis. This buffer provides a stable and consistent environment for separating and analyzing RNA molecules based on size in formaldehyde-agarose gels. It is prepared to a 10X concentration, which can be diluted to a 1X working solution for use in electrophoresis. The buffer is pH-stable around 7.2 at 25°C, offering an optimal near-neutral pH for RNA separation. It is also RNase-free to protect RNA samples from enzymatic degradation, ensuring high-quality results. 10X MOPS Buffer offers reliable ion conductivity and consistent performance for efficient RNA migration through gel matrices. Compatible with various electrophoresis systems and dyes, this buffer supports clear visualization and accurate analysis of RNA. Proper storage and handling of 10X MOPS Buffer help maintain its effectiveness and stability for consistent use in molecular biology research and diagnostic applications
#Overview
#Kit Content
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Optimal Purity and Yield
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Phase Separation
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Effective Denaturation
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Stability
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Frequently Asked Questions
Our Price
GCR-C4
₹ POR
GCR-C4A
₹ POR
GCR-E3
₹ POR
GCR-E3A
₹ POR
Product Data Sheet
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Product Brochure
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