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TRIZIN® RNA Extraction Reagent
Monophasic phenol–guanidinium reagent for high-quality total RNA extraction
Trizine™ is GCC Biotech’s high-performance, monophasic phenol–guanidinium reagent designed for the rapid isolation of high-quality total RNA from a wide variety of biological samples including animal tissues, cultured cells, bacteria, yeast, and plant material.
Functionally equivalent to widely used reagents such as TRIzol™, QIAzol® etc. and other acid-guanidinium phenol formulations, Trizine™ offers fast, reliable, and cost-effective RNA purification, yielding intact RNA suitable for RT-PCR, qPCR, Northern blotting, sequencing, and molecular diagnostics.
Using Trizine™, RNA can be isolated from as little as 10 mg tissue or 1 × 10⁴ cells, making it a versatile solution for both routine molecular biology and advanced gene expression workflows.
Key Features & Benefits
● High RNA Yield and Integrity
Optimized monophasic phenol–guanidinium chemistry ensures efficient cell lysis, protein denaturation, and RNase inactivation, producing high-integrity RNA for sensitive downstream applications.
● Compatible with:
Mammalian tissues, Cultured cells, Blood and plasma, Bacteria & yeast, Plant tissues, Virus-infected samples, Biological fluids (saliva, serum)
● Strong RNase Inactivation
Ensures preservation of RNA integrity even in challenging RNA-rich tissues (e.g., pancreas, spleen).
● Highly Cost-Effective Alternative
Delivers performance comparable to ThermoFisher TRIzol™ and Qiagen QIAzol® at a significantly reduced cost — ideal for high-throughput labs and academic institutions.
● DNA and Protein Recovery Option
As with classic TRIzol™ workflows, Trizine™ allows optional recovery of genomic DNA and protein fractions from the same sample, increasing experimental efficiency.
Applications:
- Gene expression profiling
- Viral RNA isolation (including RNA viruses in clinical research)
- RNA-Seq workflows
- Microbial and environmental RNA studies
- Diagnostic R&D
- Academic and industrial biotech research
Product :
TRIZIN® RNA Extraction Reagent
Catalog No :
G31C, G31D
#Descriptions
Trizine™ uses a proven monophasic solution of phenol and guanidinium thiocyanate to simultaneously lyse cells, inactivate RNases, and preserve RNA. After sample homogenization, the addition of chloroform induces phase separation, allowing RNA to partition into the upper aqueous phase while DNA and proteins separate into the interphase and organic layer.
RNA is then recovered by isopropanol precipitation, washed with ethanol, and resuspended in nuclease-free water. The resulting RNA is free of protein, DNA, polysaccharides, and enzymatic inhibitors.
This chemistry provides excellent RNA stability, even from RNase-rich tissues, ensuring highly reproducible gene expression data.
#Technical Specifications
| Parameter | Details |
| Reagent Type | Monophasic phenol–guanidinium thiocyanate |
| Sample Types | Animal tissues, cultured cells, bacteria, yeast, plants |
| Sample Capacity | 10–100 mg tissue or 10³–10⁷ cells per mL reagent |
| RNA Yield | Typically 5–50 µg depending on sample |
| Purity | A260/280 ratio of 1.8–2.0 |
| Storage | 15–25°C (room temperature) |
| Downstream Use | RT-PCR, qPCR, sequencing, blotting |
| Format | Ready-to-use reagent |
Advantages Over Competitor Products
✔ Comparable performance at significantly lower cost: Ideal for bulk use or high-throughput screening.
✔ Stringent RNase inactivation: Ensures intact RNA even from difficult tissues.
✔ Higher flexibility in sample input: Can handle very small or large amounts without protocol modification.
✔ Reliable performance in Indian laboratory conditions: Stable at room temperature, resistant to shipping variability.
✔ Multiplex biomolecule recovery option: RNA, DNA, and protein can be isolated sequentially from a single sample.
Why Choose Trizine™ ?
Trizine™ combines high RNA yield, excellent RNA integrity, and unmatched cost efficiency, making it the preferred reagent for RNA isolation across biotechnology labs, diagnostic R&D centres, and academic research facilities.
It delivers the same robustness as international monophasic RNA extraction reagents, while offering the convenience, support, and reliability of GCC Biotech’s Made-in-India manufacturing excellence.
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- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
