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GSure® Gel Extraction Kit
Features
- • The Gel Extraction process is completed in 30 mins
- • Highly convenient where the 3-step isolation, followed by silica membrane-based spin mini-column purification
- • High Yield where 85% of the DNA can be extracted from the agarose gel
- • Delivers an equal amount of yield on every isolation
- • Our kit can effectively remove contaminants such as genomic DNA, RNA, proteins, and other cellular debris, resulting in highly pure plasmid DNA suitable for sensitive downstream analyses. The 260:280 absorbance ratio stays around 1.79-1.83 OD which signifies that no contamination of protein and/or RNA and/or gDNA
- • Our kit is suitable for purifying DNA from size 10bp to 80bp
- • The Kit is applicable in cleaning up DNA from complex protein-rich reactions.
Applications:
- DNA Cloning
- Sequencing
- PCR Cleanup
- Gene Expression
- Site-Directed Mutagenesis
Categories:
- Cost Effective
- High Yield
- Highly Convenient
- Fast
Product :
GSure Gel Extraction Kit
Catalog No :
G4629A, G4629B, G4629C
#Descriptions
GSure® Gel Extraction Kit provides high-quality, salt-free DNA purified from 0.5–2% agarose gels, ensuring excellent recovery of DNA fragments across a wide size range. Unlike conventional gel extraction buffers that rely on chaotropic salts such as guanidine thiocyanate, the GSure® system uses a non-chaotropic gel melting chemistry, preserving DNA integrity and eliminating inhibitory carryover. This chemistry ensures that the recovered DNA retains native structural features essential for highly sensitive enzymatic reactions.
The kit utilizes a silica-based spin mini-column platform optimized for selective binding of DNA in the presence of the proprietary melting buffer. The gel melting solution efficiently solubilizes agarose and releases DNA fragments while incorporating a chromogenic indicator to visually guide the user through complete gel dissolution, ensuring reproducibility and preventing partial melting–related loss.
GSure® Gel Extraction Kit is equally effective for PCR product clean-up and can purify DNA from complex protein-rich solutions. Experimental validation demonstrates >90% recovery efficiency from PCR reactions, confirming minimal loss of nucleic acid through the workflow.
A major advantage of the GSure® system is the absence of residual chaotropic salts, which are known to inhibit ligation, polymerase activity, sequencing enzymes, and transcriptional machinery. Purified DNA obtained with this kit is fully compatible with all downstream applications, including ligation and cloning, Sanger sequencing, high-fidelity PCR amplification, qPCR, and in vitro transcription. The resulting DNA exhibits high purity, excellent functional activity, and reproducible performance, making the GSure® Gel Extraction Kit a preferred choice for molecular cloning, genetic engineering, and advanced nucleic acid manipulation workflows.
#Specification
Our GSure® Gel Extraction Kit offers high efficiency and yield in recovering DNA fragments from agarose gels while ensuring purity by effectively removing contaminants. It is versatile, accommodating various gel types and DNA staining methods, and compatible with downstream applications like PCR and sequencing. Ease of use is paramount, with user-friendly protocols and scalability to process different sample volumes. Quality control measures, including positive and negative controls, are included to ensure reliable results. The kit’s speed, storage conditions, and shelf life, as well as cost-effectiveness, are highly maintained for delivering a seamless gel extraction process.
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- Joychandipur, Bakrahat, 24 Parganas (S) 743377
- +91 9073682428
- support@gccbiotech.co.in
